Part:BBa_K157012:Design

Strep affinity tag II; Freiburg standard

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
Attention this part contains a double NgoMIV restriction site.

Source

Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008. The synthesis of this part was faulty , an additional NgoMIV restriction site was added for the normal NgoMIV restriction site.

References

Not a comprehensive list

<biblio>

1. Schmidt93 pmid=8433964

// this looks to be the original reference for the Strep-tag

1. Skerra96 pmid=8636976

// original reference for Strep-tag II

1. Voss97 pmid=9415448

// original reference for streptavidin mutant with enhanced affinity for Strep-tag and Strep-tag II. The variant was later called StrepTactin.

1. Skerra99 pmid=10796988
2. Skerra00 pmid=11036648

// history of Strep-tag system

1. Skerra07 pmid=17571060

// includes brief overview of Strep-tag system </biblio>